human mucin 16  (Santa Cruz Biotechnology)

Journal: Respirology (Carlton, Vic.)

Article Title: Pre-Treatment MMP7 Predicts Progressive Idiopathic Pulmonary Fibrosis in Antifibrotic Treated Patients.

doi: 10.1111/resp.14894

Figure Lengend Snippet: FIGURE 2 | IPF stables showed decrease in CA125 and POSTN levels 3 months post-antifibrotic treatment. Serum CA125 and POSTN levels in progressive (red) and stable (blue) at 0, 3, 6 and 12 months post-antifibrotic treatment in the (a and d) Cohort 1, (b and e) Cohort 2 and (c and f) Combined cohort. Box represents interquartile range with median and whiskers represent range. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Serum levels of matrix metalloproteinase- 7 (MMP7), periostin (POSTN), C- X- C motif ligand 13 (CXCL13), intercellular adhesion molecule- 1 (ICAM- 1), osteopontin (OPN), C- C motif ligand 18 (CCL18), surfactant protein- D (SP- D), chitinase- 3- like protein- 1 (CHI3L1) and mucin 16 (CA125) were measured by ELISA using DuoSet ELISA Kits (R&D Systems, MN, USA) according to manufacturer's instruction.

Techniques:

Journal: eLife

Article Title: Innate immune signaling in trophoblast and decidua organoids defines differential antiviral defenses at the maternal-fetal interface

doi: 10.7554/eLife.79794

Figure Lengend Snippet: ( A ) Schematic representation of the derivation of trophoblast organoids (TOs) and decidual organoids (DOs) from mid-to-late gestation placental tissue. ( B ) Representative bright-field images of TOs and DOs after passaging in complete growth medium TOM and ExM, respectively at 5 days (DO, top) or 8 days (TO, bottom) post-passaging. Scale bar, 50 µm. ( C ) Confocal microscopy in TOs immunostained for (from left) cytokeratin-19, SDC-1, SIGLEC-6, cytokeratin-19 (in green) and actin, cytokeratin-19, or MUC5AC (in red). DAPI-stained nuclei are shown in blue. Individual channels are shown in . Scale bar, 50 µm. ( D ) Confocal microscopy in DOs immunostained for (from left) cytokeratin-19, E-cadherin (E-cad), EpCAM, or MUC5AC (in green) and actin or SIGLEC6 (in red). DAPI-stained nuclei are shown in blue. Individual channels are shown in . Scale bar, 50 µm. ( E ) Levels of hCG in conditioned medium (CM) isolated from TOs (blue) or DOs (orange) as determined by Luminex. At right, over the counter (OTC) pregnancy tests for hCG in two matched TO and DO lines. ( F ) Levels of hCG in CM isolated from TOs throughout a 16-week culture period as determined by Luminex. ( G ) Levels of secreted Mucin-16 (MUC16) in CM isolated from TOs (light blue) or DOs (orange) as determined by Luminex assays. ( H ) Confocal microscopy in TOs differentiated into an EVT-enriched phenotype (Diff., right) or cultured under standard growth conditions (Undiff., left), and immunostained with HLA-G (in green). Actin is shown in red and DAPI-stained nuclei in blue. At right, black and white images of HLA-G in undifferentiated (top) or differentiated (bottom) conditions. ( I ) Levels of MMP-2 in the media of undifferentiated (undiff.) or EVT-differentiated (diff.) TOs as assessed by Luminex assays. Shown are media isolated from both stages of EVT differentiation, medium 1 (EVT m1), which contains NRG1, and medium 2 (EVT m2), without NRG1. In ( E ), ( G ), and ( I ), each symbol represents an individual CM preparation and significance was determined by Mann-Whitney U-test. ****, p<0.0001 and nd, not detected. EVT, extravillous trophoblast; hCG, human chorionic gonadotropin.

Article Snippet: Luminex assays were performed using the following kits according to the manufacturer’s instructions: Bio-Plex Pro Human Inflammation Panel 1, 37-Plex kit (171AL001M; Bio-Rad) Bio-Plex Human Chemokine Panel, 40-plex (171AK99MR2; Bio-Rad), Bio-Plex Pro Human Cytokine Screening Panel, 48-Plex kit (12007283; Bio-Rad), hCG Human ProcartaPlex Simplex Kit (Thermo Fisher Scientific, EPX010-12388-901), and CA125 (Mucin-16) Human ProcartaPlex Simplex Kit (EPX010-12437-901).

Techniques: Passaging, Confocal Microscopy, Staining, Isolation, Luminex, Cell Culture, MANN-WHITNEY

Journal: eLife

Article Title: Innate immune signaling in trophoblast and decidua organoids defines differential antiviral defenses at the maternal-fetal interface

doi: 10.7554/eLife.79794

Figure Lengend Snippet:

Article Snippet: Luminex assays were performed using the following kits according to the manufacturer’s instructions: Bio-Plex Pro Human Inflammation Panel 1, 37-Plex kit (171AL001M; Bio-Rad) Bio-Plex Human Chemokine Panel, 40-plex (171AK99MR2; Bio-Rad), Bio-Plex Pro Human Cytokine Screening Panel, 48-Plex kit (12007283; Bio-Rad), hCG Human ProcartaPlex Simplex Kit (Thermo Fisher Scientific, EPX010-12388-901), and CA125 (Mucin-16) Human ProcartaPlex Simplex Kit (EPX010-12437-901).

Techniques: Passaging, Cell Isolation, Recombinant, Plasmid Preparation, Immunofluorescence, Immunohistochemistry, Staining, Software, SYBR Green Assay